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DRX000355

FASTQ

SRA

Experiment Detail

TitleC2C12 cells transfected mir3139 targeted Chd2_Growth_H3.3 lot2
Design Descriptionnone provided
OrganismMus musculus

Library Description

NameC2C12 cells transfected mir3139 targeted Chd2_Growth_H3.3 lot2
StrategyChIP-Seq
SourceGENOMIC
SelectionChIP
LayoutSINGLE
Construction ProtocolChIP analyses were performed as described in Pasini et al. (2008). Genes Dev 22, 1345-1355. DNA from ChIP experiments was pooled and prepared for sequencing with the Illumina ChIPSeq Sample Prep kit. DNA libraries were quantified using a bioanalyzer (Agilent) and diluted to 5-10 pM. Diluted libraries were used directly for cluster generation and sequencing analysis using the Genome Analyzer IIx (Illumina) following the protocol of the manufacturer. Basecalling and mapping of the 36 bp sequences were done using illumina RTA SCS2.6 allowing up to two mismatches in the first 35 bases of a read against mouse genomes July 2007 release (mm9).

Platform

PlatformILLUMINA
Instrument ModelIllumina Genome Analyzer II

Processing

PipeSection
Step Index1
Prev Step IndexNIL
ProgramIllumina RTA SCS2.6
Version

Spot Information

Number of Reads per Spots0
Spot Length35

Read Spec

Read Index 0
Read Label
Read ClassApplication Read
Read TypeForward
Base Coord1

Navigation

Submission DRA000219 FTP
Study DRP000220
Sample DRS000321
Run DRR000600 FASTQ SRA
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