DRA SearchDRASearch

  • Search Home
  • DRA Home

DRX001287

FASTQ

SRA

Experiment Detail

TitleTranscriptome of male strobili using 454 GS FLX Titanium
Design Description
OrganismCryptomeria japonica

Library Description

NameFull-length enriched cDNA library of male strobili
StrategyFL-cDNA
SourceTRANSCRIPTOMIC
SelectioncDNA
LayoutSINGLE
Construction ProtocolThe full-length cDNA library was constructed essentially as reported previously (Seki et al. (1998) Plant J. 15:707-720; Seki et al. (2002) Science 296:141-145). The cDNAs derived from male strobili of C. japonica are inserted into a modified pBluescript vector. Plasmid DNA was digested by PI-Sce I whose site is placed near the 3'-end of a cDNA insert and then sense strand RNA was synthesized by RNA polymerase. First strand cDNA was synthesized using an oligo dT primer with resriction site of Gsu I. RNA of double stranded DNA:RNA hybrids was degraded by alkaline treatment. Second strand was synthesized using a primer annealed to a contiguous region of the 5'-end of a cDNA. Purified double strand DNA was digested by Gsu I to remove a 3' poly(A) tail. This DNA fragment was used for sequencing. The subsequent procedure was performed using GS FLX Titanium Rapid Library Preparation Kit and Rapid Library MID Adaptors Kit.

Platform

PlatformLS454
Instrument Model454 GS FLX Titanium

Processing

PipeSection (primary analysis)
Step Index1
Prev Step IndexNIL
Program454 Basecaller
Version

Spot Information

Number of Reads per Spots0
Spot Length800

Read Spec

Read Index 0
Read Label
Read ClassTechnical Read
Read TypeAdapter
Base Coord1
Read Index 1
Read Label
Read ClassTechnical Read
Read TypeAdapter
Base Coord0
Read Index 2
Read Label
Read ClassApplication Read
Read TypeForward
Base Coord16

Navigation

Submission DRA000521 FTP
Study DRP000545
Sample DRS001253
Run DRR001824 FASTQ SRA
Website policy | © DNA Data Bank of Japan