FASTQExperiment Detail
| Title | RNA-Seq analysis of developing seeds at 10-20 days after flowering of a triple mutant rice a123 (Oryza sativa L. ssp. japonica cv. Koshihikari) - replicate 1 |
| Design Description | Total RNAs were prepared from developing seeds at 10-20 days after flowering of three independent a123 plants with phenol/SDS methods. After DNase I treatment, mRNAs were purified twice with Dynabeads mRNA purification kit (LifeTechnologies). Libraries were generated with Ion total RNA-Seq v2 kit (LifeTechnologies), and sequenced by Ion Torrent PGM (LifeTechnologies) with Ion 318 Chip (LifeTechnologies). |
| Organism | Oryza sativa Japonica Group |
Library Description
| Name | a123_Transcriptome_DevelopingSeeds_1 |
| Strategy | RNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | RANDOM |
| Layout | SINGLE |
| Construction Protocol |
Platform
| Platform | ION_TORRENT |
| Instrument Model | Ion Torrent PGM |
Processing
| PipeSection | |
|---|---|
| Step Index | 1 |
| Prev Step Index | NIL |
| Program | |
| Version | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 0 |
Read Spec
| Read Index 0 | |
|---|---|
| Read Label | |
| Read Class | Application Read |
| Read Type | Forward |
| Base Coord | 1 |
