Title | RNA-Seq analysis of developing seeds at 10-20 days after flowering of a triple mutant rice a123 (Oryza sativa L. ssp. japonica cv. Koshihikari) - replicate 1 |
Design Description | Total RNAs were prepared from developing seeds at 10-20 days after flowering of three independent a123 plants with phenol/SDS methods. After DNase I treatment, mRNAs were purified twice with Dynabeads mRNA purification kit (LifeTechnologies). Libraries were generated with Ion total RNA-Seq v2 kit (LifeTechnologies), and sequenced by Ion Torrent PGM (LifeTechnologies) with Ion 318 Chip (LifeTechnologies). |
Organism | Oryza sativa Japonica Group |
Name | a123_Transcriptome_DevelopingSeeds_1 |
Strategy | RNA-Seq |
Source | TRANSCRIPTOMIC |
Selection | RANDOM |
Layout | SINGLE |
Construction Protocol |
Platform | ION_TORRENT |
Instrument Model | Ion Torrent PGM |
PipeSection | |
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Step Index | 1 |
Prev Step Index | NIL |
Program | |
Version |
Number of Reads per Spots | 0 |
Spot Length | 0 |
Read Index 0 | |
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Read Label | |
Read Class | Application Read |
Read Type | Forward |
Base Coord | 1 |