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DRX003194

FASTQ

SRA

Experiment Detail

TitleRNA-Seq analysis of developing seeds at 10-20 days after flowering of a triple mutant rice a123 (Oryza sativa L. ssp. japonica cv. Koshihikari) - replicate 1
Design DescriptionTotal RNAs were prepared from developing seeds at 10-20 days after flowering of three independent a123 plants with phenol/SDS methods. After DNase I treatment, mRNAs were purified twice with Dynabeads mRNA purification kit (LifeTechnologies). Libraries were generated with Ion total RNA-Seq v2 kit (LifeTechnologies), and sequenced by Ion Torrent PGM (LifeTechnologies) with Ion 318 Chip (LifeTechnologies).
OrganismOryza sativa Japonica Group

Library Description

Namea123_Transcriptome_DevelopingSeeds_1
StrategyRNA-Seq
SourceTRANSCRIPTOMIC
SelectionRANDOM
LayoutSINGLE
Construction Protocol

Platform

PlatformION_TORRENT
Instrument ModelIon Torrent PGM

Processing

PipeSection
Step Index1
Prev Step IndexNIL
Program
Version

Spot Information

Number of Reads per Spots0
Spot Length0

Read Spec

Read Index 0
Read Label
Read ClassApplication Read
Read TypeForward
Base Coord1

Navigation

Submission DRA000918 FTP
Study DRP000954
Sample DRS003358
Run DRR003958 FASTQ SRA
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