FASTQExperiment Detail
| Title | Transcriptome profiling of early zebrafish development |
| Design Description | Transcriptome profiling of 16 cell stage of zebrafish embryos. |
| Organism | Danio rerio |
Library Description
| Name | JKE_Drerio_rna_seq |
| Strategy | RNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | RANDOM |
| Layout | SINGLE |
| Construction Protocol | Total RNA was extracted from approximately 150 embryos per developmental stage using Trireagent (Sigma-Aldrich). The total RNA was then processed further according to the Small RNA Expression Kit (Applied Biosystems). |
Platform
| Platform | ABI_SOLID |
| Instrument Model | AB SOLiD System 3.0 |
Processing
| Base Calls | |
|---|---|
| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 50 |
Read Spec
| Read Index 0 | |
|---|---|
| Read Label | |
| Read Class | Application Read |
| Read Type | Forward |
| Base Coord | 1 |
