FASTQExperiment Detail
| Title | Profiling of Mili and Miwi2 associated piRNAs in WT, MiliDAH, Miwi2DAH and Miwi2KO from E16.5 gonadocytes. |
| Design Description | Profiling of Mili and Miwi2 associated piRNAs in WT, MiliDAH, Miwi2DAH and Miwi2KO from E16.5 gonadocytes. |
| Organism | Mus musculus |
Library Description
| Name | Mili_MiliDAH_1 |
| Strategy | RNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | size fractionation |
| Layout | SINGLE |
| Construction Protocol | MiliDAH and Miwi2DAH mice were crossed with Oct4/GFP trangenic mice to label germ cells with eGFP. To isolate E16.5 and postnatal day 7 germ cells, single cell suspensions of testis were obtained by two step enzymatic digestion and GFP-positive FACS-sorted. Mili and Miwi2 RNPs were immunoprecipitated as described in Reuter, M. et al. (2009). Small RNA libraries were generated with the protocol described in Hafner M. et al. (2008) but using adaptors suitable for sequencing on the Ilumina platform. |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina Genome Analyzer IIx |
Processing
| Base Calls | |
|---|---|
| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 36 |
Read Spec
| Read Index 0 | |
|---|---|
| Read Label | |
| Read Class | Application Read |
| Read Type | Forward |
| Base Coord | 1 |
