Experiment Detail
| Title | Illumina HiSeq 2000 sequencing; RNAseq analysis of wild type dark grown PSB-D cell cultures |
| Design Description | RNAseq analysis of wild type dark grown PSB-D cell cultures |
| Organism | Arabidopsis thaliana |
Library Description
| Name | AXR1_DMSO_R3 |
| Strategy | RNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | RANDOM |
| Layout | SINGLE |
| Construction Protocol | PSB-D cell cultures were grown in continous darkness in MSMO medium. RNeasy Plant Mini Kit (Qiagen) and DNase I treated (Promega) Preparation of strand-specific cDNA libraries: Purification of poly-A containing mRNA molecules; mRNA fragmentation; Random primed cDNA synthesis (strand specific); Adapter ligation and adapter specific PCR amplification. |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina HiSeq 2000 |
Processing
| Base Calls | |
|---|---|
| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 0 |
