Title | Illumina HiSeq 2000 sequencing; Determining the transcriptome of an AdeRS deletion mutant in Acinetobacter baumannii AYE |
Design Description | Determining the transcriptome of an AdeRS deletion mutant in Acinetobacter baumannii AYE |
Organism | Acinetobacter baumannii AYE |
Name | AYE_20 |
Strategy | RNA-Seq |
Source | TRANSCRIPTOMIC |
Selection | RANDOM |
Layout | SINGLE |
Construction Protocol | Cells were grown to mid-logarithmic phase at 37C in Luria Bertani broth RNA was extracted using the Qiagen RNAprotecta Bacteria Reagent and RNeasy Mini kit and DNase treated using an 435 Ambion TURBO DNA-freea RNA libraries were prepared by ARK genomics as per their standard protocol |
Platform | ILLUMINA |
Instrument Model | Illumina HiSeq 2000 |
Base Calls | |
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Sequence Space | |
Base Caller |
Number of Reads per Spots | 0 |
Spot Length | 0 |