Experiment Detail
| Title | Illumina HiSeq 2000 sequencing; Continuous CRISPR spacer acquisition in STSV2-infected crenarchaeon Sulfolobus islandicus REY15A over a 6-day period |
| Design Description | Continuous CRISPR spacer acquisition in STSV2-infected crenarchaeon Sulfolobus islandicus REY15A over a 6-day period |
| Organism | Saccharolobus solfataricus P2 |
Library Description
| Name | SCV 7 |
| Strategy | RNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | RANDOM |
| Layout | SINGLE |
| Construction Protocol | 1x Sulfolobus medium supplemented with 0.2% sucrose, 0.2% vitamin-free casamino acids (Difco Vitamin Assay, BD), vitamin solution (Zillig et al., 1993), 0.02 mg/ml uracil TRIzol extraction, isopropanol precipitation containing 15ul glycogen (GlycoBlue), DNaseI treatment (Ambion) Total RNA library, stranded, rRNA depleted |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina HiSeq 2000 |
Processing
| Base Calls | |
|---|---|
| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 0 |
