Experiment Detail
| Title | Illumina MiSeq paired end sequencing; Continuous CRISPR spacer acquisition in STSV2-infected crenarchaeon Sulfolobus islandicus REY15A over a 6-day period |
| Design Description | Continuous CRISPR spacer acquisition in STSV2-infected crenarchaeon Sulfolobus islandicus REY15A over a 6-day period |
| Organism | Saccharolobus solfataricus P2 |
Library Description
| Name | TYS 15 (paired) |
| Strategy | RNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | RANDOM |
| Layout | PAIRED |
| Orientation | |
| Nominal Length | 250 |
| Nominal Sdev | 25 |
| Construction Protocol | 1x Sulfolobus medium supplemented with 0.2% trypton, 0.1% yeast extract, 0.2% sucrose, 0.02 mg/ml uracil TRIzol extraction, isopropanol precipitation containing 15ul glycogen (GlycoBlue), DNaseI treatment (Ambion) Total RNA library, stranded, rRNA depleted |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina MiSeq |
Processing
| Base Calls | |
|---|---|
| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 70 |
Read Spec
| Read Index 0 | |
|---|---|
| Read Label | |
| Read Class | Application Read |
| Read Type | Forward |
| Base Coord | 1 |
| Read Index 1 | |
| Read Label | |
| Read Class | Application Read |
| Read Type | Reverse |
| Base Coord | 36 |
