Experiment Detail
| Title | Illumina HiSeq 2000 sequencing; Morphological, electrophysiological and transcriptomic profiling of single neurons using Patch-seq |
| Design Description | Morphological, electrophysiological and transcriptomic profiling of single neurons using Patch-seq |
| Organism | Mus musculus |
Library Description
| Name | Int_52 |
| Strategy | RNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | RANDOM |
| Layout | SINGLE |
| Construction Protocol | Single-cell RNA was collected following whole-cell patch clamp recording (Jiang et al., Nature Neuroscience, 2013) by applying negative pressure to the glass micropipette (Cadwell et al., in press). Picelli et al, Full-length RNA-seq from single cells using Smart-seq2, Nature Protocols, 2014 |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina HiSeq 2000 |
Processing
| Base Calls | |
|---|---|
| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 0 |
