Experiment Detail
| Title | NextSeq 500 sequencing; RNA-seq in HUVECs overexpressing Sox18 and treated with Sm4 |
| Design Description | RNA-seq in HUVECs overexpressing Sox18 and treated with Sm4 |
| Organism | Homo sapiens |
Library Description
| Name | Sox18 o.e. + Sm4 #1 |
| Strategy | RNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | RT-PCR |
| Layout | SINGLE |
| Construction Protocol | HUVECs were obtained from LONZA and grown according to suppliers recommendations. Cells were cultured in EBM2 media, supplemented with EGM2. Transfection (7 hours) was performed using Roche X-tremegene DNA transfection reagent in 0.4% FBS media. Directly following transfection, cells were stimulated (14 hours) with small molecule (or DMSO), in 0.4% FBS media. RNA was extracted using RNeasy mini kit (Qiagen). Samples (>100 ng RNA) were processed for whole transcriptome sequencing using TruSeq stranded total RNA library prep kit (Ribo‐Zero GOLD, Illumina). |
Platform
| Platform | ILLUMINA |
| Instrument Model | NextSeq 500 |
Processing
| Base Calls | |
|---|---|
| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 0 |
Read Spec
Navigation
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Submission | ERA568750 |
 FTP
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Study | ERP014449 | ||
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Sample | ERS1073751 | ||
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Run | ERR1299350 |
FASTQ
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SRA
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