Title | Illumina HiSeq 2000 paired end sequencing; RNA-Seq analysis in bovine muscle |
Design Description | RNA-Seq analysis in bovine muscle |
Organism | Bos taurus |
Name | 1339 |
Strategy | RNA-Seq |
Source | TRANSCRIPTOMIC |
Selection | size fractionation |
Layout | PAIRED |
Orientation | |
Nominal Length | 200 |
Nominal Sdev | 50 |
Construction Protocol | muscle biopsies were obtained at the slaughterhouse Qiagen RNAeasy Midi protocol 4 ug of total RNA were used with Illumina TruSeq RNA sample preparation kit to make the paired-end RNA-Seq libraries; size selection of 200+/-25 bp fragments |
Platform | ILLUMINA |
Instrument Model | Illumina HiSeq 2000 |
Base Calls | |
---|---|
Sequence Space | |
Base Caller |
Number of Reads per Spots | 0 |
Spot Length | 202 |
Read Index 0 | |
---|---|
Read Label | |
Read Class | Application Read |
Read Type | Forward |
Base Coord | 1 |
Read Index 1 | |
Read Label | |
Read Class | Application Read |
Read Type | Reverse |
Base Coord | 102 |