Experiment Detail
| Title | Illumina HiSeq 2000 paired end sequencing; Drosophila Genetic Reference Panel (DGRP) F1 RNA-seq - a comparison to 3prime-tag-seq variance decomposition analysis |
| Design Description | Drosophila Genetic Reference Panel (DGRP) F1 RNA-seq - a comparison to 3prime-tag-seq variance decomposition analysis |
| Organism | Drosophila melanogaster |
Library Description
| Name | QTLF1_m765p517_24h_rep1_2 |
| Strategy | RNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | Oligo-dT |
| Layout | PAIRED |
| Orientation | |
| Nominal Length | 200 |
| Nominal Sdev | 25 |
| Construction Protocol | Collect virgins from the maternal line, crossing with the males collected from the paternal line. Keep the flies in small cages and feed on apple plates with yeast. Staged 2 hr populations of embryos were collected and aged at 25 degr till the required stage of development. The collected embryos were dechorionated using 50% bleach, rinsed with water, blotted dry and snap freeze in liquid nitrigen. The embryos are kept in -80 degr until use for RNA extraction. Total RNA extracted from frozen Drosophila embryos. Then use Oligo-dT beads to extract mRNA. Libraries were prepared following instructions of the NEBNext ultra directional RNA library prep kit for Illumina sequencing (NEB). |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina HiSeq 2000 |
Processing
| Base Calls | |
|---|---|
| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 246 |
Read Spec
| Read Index 0 | |
|---|---|
| Read Label | |
| Read Class | Application Read |
| Read Type | Forward |
| Base Coord | 1 |
| Read Index 1 | |
| Read Label | |
| Read Class | Application Read |
| Read Type | Reverse |
| Base Coord | 124 |
Navigation
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Submission | ERA612182 |
 FTP
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Study | ERP015406 | ||
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Sample | ERS1137515 | ||
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Run | ERR1395645 |
FASTQ
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SRA
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