Experiment Detail
| Title | Illumina HiSeq 2000 paired end sequencing; Acquired TET2 mutation-linked clonal hematopoiesis in FPD/AML patient led to the development of pre-leukemic clone resulting independently in T2-ALL and AML-M0. |
| Design Description | Acquired TET2 mutation-linked clonal hematopoiesis in FPD/AML patient led to the development of pre-leukemic clone resulting independently in T2-ALL and AML-M0. |
| Organism | Homo sapiens |
Library Description
| Name | SC_LAM |
| Strategy | WXS |
| Source | GENOMIC |
| Selection | other |
| Layout | PAIRED |
| Orientation | |
| Nominal Length | 175 |
| Nominal Sdev | 25 |
| Construction Protocol | total peripheral blood cells were collected, AML-M0 blast cells represent 87% of total population DNA was extracted using Qiagen Kit The fragment ends were repaired, “A” bases were added ends of the DNA fragments and adapters were ligated to the fragments. The adapter-ligated library was amplified using 4 to 6 cycles of PCR. 500 nanograms of the sample library were hybridized with the SureSelect biotinylated RNA library for 24 hours. Bound DNA was purified using streptavidin coated magnetic beads |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina HiSeq 2000 |
Processing
| Base Calls | |
|---|---|
| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 152 |
Read Spec
| Read Index 0 | |
|---|---|
| Read Label | |
| Read Class | Application Read |
| Read Type | Forward |
| Base Coord | 1 |
| Read Index 1 | |
| Read Label | |
| Read Class | Application Read |
| Read Type | Reverse |
| Base Coord | 77 |
Navigation
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Submission | ERA613084 |
 FTP
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Study | ERP015451 | ||
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Sample | ERS1138938 | ||
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Run | ERR1398267 |
FASTQ
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SRA
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