Experiment Detail
| Title | Illumina HiSeq 2000 paired end sequencing; RNA-seq of sh2-H2AFJ and sh-NoTarget in WI-38hTERT cells induced into senescence by etoposide |
| Design Description | RNA-seq of sh2-H2AFJ and sh-NoTarget in WI-38hTERT cells induced into senescence by etoposide |
| Organism | Homo sapiens |
Library Description
| Name | shSEN4_1_p |
| Strategy | RNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | size fractionation |
| Layout | PAIRED |
| Orientation | |
| Nominal Length | 300 |
| Nominal Sdev | 100 |
| Construction Protocol | MEM+10%FBS+glutamine+pyruvate+non-essential amino acids in 5%02 + 5%C02 etoposide 20 µM Ribo-Zero Magnetic Gold Kip (Epicentre) Illumina TruSeq adapters |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina HiSeq 2000 |
Processing
| Base Calls | |
|---|---|
| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 202 |
Read Spec
| Read Index 0 | |
|---|---|
| Read Label | |
| Read Class | Application Read |
| Read Type | Forward |
| Base Coord | 1 |
| Read Index 1 | |
| Read Label | |
| Read Class | Application Read |
| Read Type | Reverse |
| Base Coord | 102 |
Navigation
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Submission | ERA673995 |
 FTP
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Study | ERP016433 | ||
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Sample | ERS1245274 | ||
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Run | ERR1527164 |
FASTQ
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SRA
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