Experiment Detail
| Title | Illumina HiSeq 1500 sequencing; Small RNA and total RNA sequencing of C. elegans with mutations in the hrde-1 and emb-4 genes |
| Design Description | Small RNA and total RNA sequencing of C. elegans with mutations in the hrde-1 and emb-4 genes |
| Organism | Caenorhabditis elegans |
Library Description
| Name | SX2000.3 RNA_s |
| Strategy | RNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | RANDOM |
| Layout | SINGLE |
| Construction Protocol | Animals were harvested and washed 3X in M9 buffer. Synchronised animals were grown to young adult stage at 20C on HB101 seeded NGM plates. Settled animals were mixed with Trisure reagent, bead beaten using zirconia beads, and the RNA is extracted using chloroform. For total RNA sequencing, Illumina Ribozero kit is used to remove ribosomal RNA from 1ug of total RNA prior to library preparation. RNA sequencing libraries are prepared using NEB Next Ultra library preparation kit. |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina HiSeq 1500 |
Processing
| Base Calls | |
|---|---|
| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 0 |
Read Spec
Navigation
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Submission | ERA674225 |
 FTP
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Study | ERP016462 | ||
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Sample | ERS1247855 | ||
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Run | ERR1530697 |
FASTQ
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SRA
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