Experiment Detail
| Title | Illumina HiSeq 2500 paired end sequencing; human SU-DHL-5 and TMD-8 B-lymphome cell line subclones |
| Design Description | human SU-DHL-5 and TMD-8 B-lymphome cell line subclones |
| Organism | Homo sapiens |
Library Description
| Name | Sample 4_p |
| Strategy | WXS |
| Source | GENOMIC |
| Selection | Hybrid Selection |
| Layout | PAIRED |
| Orientation | |
| Nominal Length | 101 |
| Nominal Sdev | 0.0E0 |
| Construction Protocol | Cell lines were FACS-sorted and single cell clones were picked and phenotyped. Growth of sorted cell clones was observed over a four-week period by microscopy. Fragmentation of 100 ng purified genomic DNA (gDNA) in 55 l Tris-EDTA buffer was done on Covaris S2. Fragmentation of 100 ng purified genomic DNA (gDNA) in 55 l Tris-EDTA buffer was done on Covaris S2. After library preparation from 100 ng of fragmented gDNA using Agilent SureSelectXT v5+UTR (75 Mb), libraries were purified, size validated and prepared for sequencing according to the manufacturer's protocols. Libraries were sequenced on Illumina HiSeq2500 using TruSeq SBS Kit v3-HS (2x101, paired-end run). |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina HiSeq 2500 |
Processing
| Base Calls | |
|---|---|
| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 200 |
Read Spec
| Read Index 0 | |
|---|---|
| Read Label | |
| Read Class | Application Read |
| Read Type | Forward |
| Base Coord | 1 |
| Read Index 1 | |
| Read Label | |
| Read Class | Application Read |
| Read Type | Reverse |
| Base Coord | 101 |
Navigation
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Submission | ERA676255 |
 FTP
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Study | ERP016553 | ||
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Sample | ERS1262247 | ||
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Run | ERR1544603 |
FASTQ
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SRA
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