Experiment Detail
| Title | NextSeq 500 paired end sequencing; Whole-islet RNA-sequencing analysis of human pancreas and type 2 diabetes. |
| Design Description | Whole-islet RNA-sequencing analysis of human pancreas and type 2 diabetes. |
| Organism | Homo sapiens |
Library Description
| Name | HP1506401_wholeislet_p |
| Strategy | RNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | RANDOM |
| Layout | PAIRED |
| Orientation | |
| Nominal Length | 500 |
| Nominal Sdev | 200 |
| Construction Protocol | Human primary islets were purchased from Prodo Laboratories Inc (Irvine, CA, USA) Qiagen RNeasy microkit with on-column DNase digestion (Qiagen, Hombrechtikon, Swizerland) Illumina TruSeq Stranded mRNA Library prep kit following the manufacturer’s recommendations. |
Platform
| Platform | ILLUMINA |
| Instrument Model | NextSeq 500 |
Processing
| Base Calls | |
|---|---|
| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 70 |
Read Spec
| Read Index 0 | |
|---|---|
| Read Label | |
| Read Class | Application Read |
| Read Type | Forward |
| Base Coord | 1 |
| Read Index 1 | |
| Read Label | |
| Read Class | Application Read |
| Read Type | Reverse |
| Base Coord | 36 |
Navigation
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Submission | ERA705445 |
 FTP
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Study | ERP017123 | ||
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Sample | ERS1348459 | ||
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Run | ERR1629984 |
FASTQ
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SRA
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