Experiment Detail
| Title | Illumina HiSeq 2000 sequencing; Single-cell RNA-seq analysis of human pancreas from healthy individuals and type 2 diabetes patients |
| Design Description | Single-cell RNA-seq analysis of human pancreas from healthy individuals and type 2 diabetes patients |
| Organism | Homo sapiens |
Library Description
| Name | HP1502401_J8_s |
| Strategy | RNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | RANDOM |
| Layout | SINGLE |
| Construction Protocol | Human primary islets (85–95% pure) were purchased from Prodo Laboratories Inc (Irvine, CA, USA) and cultured for 4 days in Prodo Islet Media Standard PIM(S) to recover after arrival. Islets were dissociated into single-cell suspension and viable individual cells were distributed by FACS into 384-well plates containing lysis buffer. Spike-in RNA from the External RNA Controls Consortium (ERCC) (Ambion, Life Technologies) was added to each well in 1:40 000 dilution. Single-cell RNA-seq libraries were generated as decribed in Picelli et al., 2014, Full-length RNA-seq from single cells using Smart-seq2, Nature Protocols. |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina HiSeq 2000 |
Processing
| Base Calls | |
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| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 0 |
Read Spec
Navigation
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Submission | ERA705448 |
 FTP
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Study | ERP017126 | ||
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Sample | ERS1348799 | ||
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Run | ERR1630342 |
FASTQ
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SRA
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