Experiment Detail
| Title | Illumina HiSeq 2500 sequencing; Akkermanisa muciniphila response to oxygen exposure |
| Design Description | Akkermanisa muciniphila response to oxygen exposure |
| Organism | Akkermansia muciniphila ATCC BAA-835 |
Library Description
| Name | 8_GTGGCC_L001_R1_001_BHAUDVADXX.filt.fastq.gz_s |
| Strategy | RNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | RANDOM |
| Layout | SINGLE |
| Construction Protocol | In fermentor on mucus medium Total RNA was isolated by a method combining the Trizol® Reagent and the RNeasy Mini kit (QIAGEN GmbH, Hilden, Germany) Library construction for whole-transcriptome sequencing (RNA-Seq) was done by ScriptSeq™ v2 RNA-Seq Library Preparation Kit in combination with ScriptSeq™ Index PCR primers (Epicentre, Madison, WI, USA) according to the manufacturer’s instructions |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina HiSeq 2500 |
Processing
| Base Calls | |
|---|---|
| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 0 |
Read Spec
Navigation
|
Submission | ERA713501 |
 FTP
|
|
|
|
Study | ERP017361 | ||
|
Sample | ERS1370389 | ||
|
Run | ERR1662975 |
FASTQ
|
SRA
|