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ERX1749523

FASTQ

SRA

Experiment Detail

Title454 GS FLX Titanium sequencing; Evidence of Late Pleistocene origin of Astyanax mexicanus cavefish
Design DescriptionEvidence of Late Pleistocene origin of Astyanax mexicanus cavefish
OrganismAstyanax mexicanus

Library Description

NameSFN2_454_s
StrategyRNA-Seq
SourceTRANSCRIPTOMIC
SelectionRANDOM PCR
LayoutSINGLE
Construction ProtocolHyphessobrycon anisitsi and Astyanax mexicanus Pachon cave and Texas surface morphs were used. Sample collection included embryos and larvae encompassing stages from 10hpf (hours post-fertilisation) to 17dpf (days post-fertilisation), as well as dissected brains and olfactory epithelia from adults. Samples were immersed in Trizol (Invitrogen) and freezed at -80°C. Total RNA was extracted from the samples according to the manufacturer's instructions for Trizol Reagent (Invitrogen) mRNA were purified from total RNA (FastTrack mRNA isolation kit (Thermo Fischer)), fragmented (NEBNext Magnesium RNA fragmentation module, NEB) and treated with the TruSeq small RNA library preparation Kit (Illumina) according to the manufacturer recommendations. Final libraries were purified with AMPureXP kit (Beckman Coulter) instead of gel purification to recover all fragments. Library quality has been assessed on a bioanalyzer instrument (Agilent).

Platform

PlatformLS454
Instrument Model454 GS FLX Titanium

Processing

Base Calls
Sequence Space
Base Caller

Spot Information

Number of Reads per Spots0
Spot Length0

Read Spec

Navigation

Submission ERA732768 FTP
Study ERP017705
Sample ERS1403031
Run ERR1679842 FASTQ SRA
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