Experiment Detail
| Title | Illumina HiSeq 2500 sequencing; A genetics screen highlights emerging roles for CPL3, RST1 and URT1 in RNA metabolism and silencing (small RNA profiling part) |
| Design Description | A genetics screen highlights emerging roles for CPL3, RST1 and URT1 in RNA metabolism and silencing (small RNA profiling part) |
| Organism | Arabidopsis thaliana |
Library Description
| Name | sgi3_R3_s |
| Strategy | ncRNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | size fractionation |
| Layout | SINGLE |
| Construction Protocol | 17-day-old (DAS) shoot tissues of plants were harvested. Shoot tissues, consist of stems including the leaves, were harvested and immediately stored in liquid nitrogen. Seedlings were grown on half strength (1/2) MS medium containing 1% sucrose at 21˚C under a 16 h/8 h day/night condition. RNA samples for small RNA sequencing analyses were extracted from 17-day-old shoot tissues of plants by the miRNeasy Mini Kit (Qiagen). Small RNAs (<50 nt) were gel-purified to build the libraries with TruSeq small RNA sample prep kit (illumina). |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina HiSeq 2500 |
Processing
| Base Calls | |
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| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 0 |
Read Spec
Navigation
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Submission | ERA1852310 |
 FTP
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Study | ERP114927 | ||
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Sample | ERS3378906 | ||
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Run | ERR3288743 |
FASTQ
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SRA
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