FASTQExperiment Detail
| Title | Illumina HiSeq 2000 sequencing; RNA-seq of H3.3 knockout and wild type chicken DT40 cells |
| Design Description | RNA-seq of H3.3 knockout and wild type chicken DT40 cells |
| Organism | Gallus gallus |
Library Description
| Name | H3.3-3 |
| Strategy | RNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | cDNA |
| Layout | SINGLE |
| Construction Protocol | Chicken DT40 cells were grown in RPMI-1640 with 7% FBS, 3% chicken serum and supplemented with pen/strep at 37C with 10% CO2. The source (starting sample for the experiment) were wild type and H3.3 knockout DT40 cells. RNA was extracted using an RNeasy Mini Kit (Qiagen). The RNA quality and quantity was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies). Libraries were constructed by BGI (Beijing Genomics Institute) |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina HiSeq 2000 |
Processing
| Base Calls | |
|---|---|
| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 0 |
