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ERX528579

FASTQ

SRA

Experiment Detail

TitleIllumina Genome Analyzer IIx sequencing; MYCN regulated miRNAs
Design DescriptionMYCN regulated miRNAs
OrganismHomo sapiens

Library Description

NameControl_A_TGACCA_L001_R1_001
StrategymiRNA-Seq
SourceTRANSCRIPTOMIC
Selectionsize fractionation
LayoutSINGLE
Construction ProtocolUn-induced SY5Y-MYCN cells and cells treated with 1ug/ml Doxycycline (Sigma) to induce MYCN overexpression, for 1h, 4h 24h were used. For the time-course treatment start times were staggered so all samples were lysed simultaneously. Total mRNA was extracted using TRI Reagent (Sigma-Aldrich) according to manufacturer’s protocol, and DNA was digested with DNA-free Kit (Applied Biosystems). miRNA libraries (SY5Y-MYCN; un-induced, 1h, 4h and 24h MYCN induction) were prepared using TruSeq small RNA sample preparation kit (Illumina).

Platform

PlatformILLUMINA
Instrument ModelIllumina Genome Analyzer IIx

Processing

Base Calls
Sequence Space
Base Caller

Spot Information

Number of Reads per Spots0
Spot Length0

Read Spec

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Submission ERA336488 FTP
Study ERP006427
Sample ERS513157
Run ERR569587 FASTQ SRA
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