Sample Detail

TitleE-MTAB-4897:Sample 3
DescriptionProtocols: Human umbilical vein endothelial cells (HUVEC) were isolated from umbilical cords from four different donors, as previously described (Cooke, B.M., Usami, S., Perry, I., and Nash, G.B. (1993). A Simplified Method for Culture of Endothelial-Cells and Analysis of Adhesion of Blood-Cells under Conditions of Flow. Microvasc Res 45, 33-45) Cells were maintained in Medium 199 (M199, Invitrogen) containing 20% fetal calf serum, 28μg/ml gentamycin, 2.5μg/ml amphotericin B, 1ng/ml epidermal growth factor and 1μg/ml hydrocortisone (all from Sigma) for 48 hours prior to processing. HUVEC cultures isolated using this method were 96-98% pure, determined by positive staining by flow cytometry of CD105, CD31 and vWF and the expression of elevated levels of intracellular adhesion molecule (ICAM-1) and E-selectin following stimulation with the inflammatory cytokine interleukin-1β. Total HUVEC RNA was isolated using the RNeasy mini kit with QIAshredder (Qiagen) according to the manufacturer's instructions. RNA integrity number was >8.0 for all samples. Libraries were prepared using Illumina TruSeq RNA Library Preparation Kit v2, with poly-A enrichment

Organism Info

Taxon ID9606
Common Name
Scientific NameHomo sapiens
Anonymized Name
Individual Name