Sample Detail

Title454 Sequencing of Pyrenophora semeniperda (cDNA library)
DescriptionFungal cultures and tissue were prepared as described by Boose et al.(2011). RNA was isolated from two P. semeniperda isolates using the ZR Fungal/Bacterial RNA MiniPrepTM from Zymo Research (Zymo Research Corporation, Orange, CA) and stored at -80* C;RNA was collected from multiple tissue types including mycelium, fingers, and conidia from P.semeniperda isolates including CCB06 and the WRK0 isolate, collected from a site at Skull Valley,UT. RNA quality and integrity was assessed for each extraction using the RNA Nano 6000 kit from Agilent Technologies and the 2100 Bioanalyzer Expert software (Agilent Technologies, Santa Clara,CA). RNA quantity was measured with the TBS-380 Mini-Fluorometer (Turner Biosystems,Sunnyvale, CA) in combination with the RiboGreen RNA quantitation reagent (Molecular Probes,Eugene, OR). Boose, D., Harrison, S., Clement, S. & Meyer, S. (2011). Population genetic structure of the seed pathogen Pyrenophora semeniperda on Bromus tectorum in western North America. Mycologia 103, 85-93.

Organism Info

Taxon ID91370
Common Name
Scientific NamePyrenophora seminiperda
Anonymized Name
Individual Name