Sample Detail

TitlePea aphid male morphs
DescriptionAphids were reared on broad bean Vicia faba at low density (less than five individuals per plant) to prevent the production of winged morphs. The production of sexual individuals was initiated by transferring larvae from a 16h to a 12h photoperiod at the same temperature of 18ÁC. Two generations later, sexual females and males were observed. A total of 20 adult females were then directly frozen into liquid nitrogen. RNA extractions were then performed using the SV Total RNA Isolation System (Promega) according to manufacturerÍs instructions. 4 separate RNA extractions of 5 adult individuals were performed. RNA quality was checked on Bioanalyzer (Agilent) and quantified on Nanodrop (Thermo Scientific). Two samples made of a pool of 2 µg of two of the four independent RNA extractions were generated and were subsequently sent to GATC. Company for RNA paired-end sequencing.

Organism Info

Taxon ID7029
Common Name
Scientific NameAcyrthosiphon pisum
Anonymized Name
Individual Name