| Title | Microbiota of incubated salivary microbiome |
| Study Type | Other |
| Abstract |
Salivary samples of 2 healthy subjects were collected at 10:30 and a part of them were incubated to be sampled every hour until 16:30. Fresh salivary samples were also collected concurrently. The 16S rRNA gene hypervariable V1-V2 regions were PCR-amplified using universal primers (a barcoded 27Fmod .. [more]and 338R) and, multiplexed amplicon pyrosequencing was carried out using 454 GS FLX Titanium or 454 GS JUNIOR (Roche Applied Science). [less]
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| Description |
Salivary samples of 2 healthy subjects were collected at 10:30 and a part of them were incubated to be sampled every hour until 16:30. Fresh salivary samples were also collected concurrently. The 16S rRNA gene hypervariable V1-V2 regions were PCR-amplified using universal primers (a barcoded 27Fmod .. [more]and 338R) and, multiplexed amplicon pyrosequencing was carried out using 454 GS FLX Titanium or 454 GS JUNIOR (Roche Applied Science). [less]
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| Center Name | UT_COB |
FTP
