Study Detail

TitleEvaluation of 16S RNA gene amplicon sequencing analyses
Study TypeOther
Abstract We adopt the direct PCR strategy amplifying 16S rRNA genes from bacterial cell suspensions without DNA purification. Our results showed that differences in cell wall morphology significantly affected the direct PCR efficiency and the sequencing data. Notably, mechanical cell disruption preceding the .. [more]
Description We adopt the direct PCR strategy amplifying 16S rRNA genes from bacterial cell suspensions without DNA purification. Our results showed that differences in cell wall morphology significantly affected the direct PCR efficiency and the sequencing data. Notably, mechanical cell disruption preceding the .. [more]
Center NameTOKAI