| Title | Gut microbiota convert HMCA into HMPA in the intestine |
| Study Type | Other |
| Abstract |
Male C57BL/6J mice were divided into two groups of similar average body weight [HFD-fed group and 4-hydroxy-3-methoxycinnamic acid (HMCA)-fed group to HFD] for 12 weeks in a factorial design. Cecums were collected at after treatments. Cecal DNA was extracted from frozen samples using the FastDNA SPI .. [more]N Kit for Feces by following the manufacturer's instructions. Partial 16S rRNA gene sequences were amplified targeting the hypervariable regions V4 using 515F/806R primers. The amplicons were sequenced using an Illumina MiSeq with MiSeq Reagent 222 kit V3. The libraries were prepared following the Illumina protocol for 8pM: 'Preparing Libraries for Sequencing on the MiSeq'. [less]
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| Description |
Male C57BL/6J mice were divided into two groups of similar average body weight [HFD-fed group and 4-hydroxy-3-methoxycinnamic acid (HMCA)-fed group to HFD] for 12 weeks in a factorial design. Cecums were collected at after treatments. Cecal DNA was extracted from frozen samples using the FastDNA SPI .. [more]N Kit for Feces by following the manufacturer's instructions. Partial 16S rRNA gene sequences were amplified targeting the hypervariable regions V4 using 515F/806R primers. The amplicons were sequenced using an Illumina MiSeq with MiSeq Reagent 222 kit V3. The libraries were prepared following the Illumina protocol for 8pM: 'Preparing Libraries for Sequencing on the MiSeq'. [less]
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| Center Name | TOKYO_AGRITECH |
FTP
