| Title | HMPA intake modifies the changes of HFD-associated gut microbial composition |
| Study Type | Other |
| Abstract |
Male C57BL/6J mice were divided into two groups of similar average body weight [cellulose-fed (control) group and 3-(4-hydroxy-3-methoxyphenyl)propionic acid (HMPA)-fed group] for 12 weeks in a factorial design. Cecums were collected at after treatments. Cecal DNA was extracted from frozen samples u .. [more]sing the FastDNA SPIN Kit for Feces by following the manufacturer's instructions. Partial 16S rRNA gene sequences were amplified targeting the hypervariable regions V4 using 515F/806R primers. The amplicons were sequenced using an Illumina MiSeq with MiSeq Reagent 222 kit V3. The libraries were prepared following the Illumina protocol for 8pM: 'Preparing Libraries for Sequencing on the MiSeq'. [less]
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| Description |
Male C57BL/6J mice were divided into two groups of similar average body weight [cellulose-fed (control) group and 3-(4-hydroxy-3-methoxyphenyl)propionic acid (HMPA)-fed group] for 12 weeks in a factorial design. Cecums were collected at after treatments. Cecal DNA was extracted from frozen samples u .. [more]sing the FastDNA SPIN Kit for Feces by following the manufacturer's instructions. Partial 16S rRNA gene sequences were amplified targeting the hypervariable regions V4 using 515F/806R primers. The amplicons were sequenced using an Illumina MiSeq with MiSeq Reagent 222 kit V3. The libraries were prepared following the Illumina protocol for 8pM: 'Preparing Libraries for Sequencing on the MiSeq'. [less]
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| Center Name | TOKYO_AGRITECH |
FTP
