Study Detail

TitleGenome editing in human cells by Type I-E CRISPR-Cas3
Study TypeOther
Abstract We demonstrate that type I-E CRISPR-Cas3, which is composed of Escherichia coli, mediates distinct DNA cleavage activity in human HEK293T cells. Notably, Cas3 predominantly triggered several thousand base pair deletions upstream of the protospacer adjacent motif (PAM), without prominent off-target .. [more]
Description We demonstrate that type I-E CRISPR-Cas3, which is composed of Escherichia coli, mediates distinct DNA cleavage activity in human HEK293T cells. Notably, Cas3 predominantly triggered several thousand base pair deletions upstream of the protospacer adjacent motif (PAM), without prominent off-target .. [more]
Center NameOSAKAMED