Study Detail

TitleMetaHit Single Cell WGA
Study TypeWhole Genome Sequencing
Abstract Abstract: In order to facilitate sequencing of uncultured phylotypes from the human gut microbiota it was first necessary to isolate individual bacterial cells via Laser microdissection microscopy. Isolated individual cells were lysed by heat treatment in a stabilising sample buffer. Genomic DNA re .. [more]
Description Project Description: Aim: The Purpose of the project is whole genome sequencing of bacterial DNA derived from single cells isolated from environmental samples, the genomic DNA amplified by multiple displacement amplification (MDA) prior to sequencing. For initial proof of concept studies cells will .. [more]
Center NameSC