Study Detail

TitleMutRenSeq_Sr45
Study TypeOther
Abstract Wheat accession CS1D5406 is carrying the resistance gene Sr45. Seeds were mutated using EMS. Screening with pathogen revealed mutants that lost resistance. We enriched genomic DNA of wildtype and 6 mutants with a bait library specific for NB-LRRs and sequenced on Illumina platform.
Description Wheat accession CS1D5406 is carrying the resistance gene Sr45. Seeds were mutated using EMS. Screening with pathogen revealed mutants that lost resistance. We enriched genomic DNA of wildtype and 6 mutants with a bait library specific for NB-LRRs and sequenced on Illumina platform.
Center NameJohn Innes Centre