Study Detail

Study TypeTranscriptome Analysis
Abstract Paired-end sequence data from the IlluminaHiSeq was prepared from RNA of zebrafish hesx1 embryos at 80% epiboly for transcriptional profiling
Description otal RNA was extracted from zebrafish embryos at 80% epiboly and genotyped for hesx1 to identify wild type, heterozygous and homozygous knockout embryos. The 3' ends of fragmented RNA was pulled down using polyT oligos attached to magnetic beads, reverse transcribed, made into Illumina libraries and .. [more]
Center NameThe Wellcome Trust Sanger Institute