Study Detail

TitleOverexpression_of_prl3_in_zebrafish_embryos
Study TypeTranscriptome Analysis
Abstract Paired-end sequence data from the IlluminaHiSeq was prepared from RNA of zebrafish embryos over expressing prl3 by use of outcrossed tg(actin:prl3) fish comparing prl3 overexpression to wild type siblings
Description Over expression of prl3 by use of outcrossed tg(actin:prl3) fish comparing prl3 overexpression to wild type siblings. The 3' ends of fragmented RNA was pulled down using polyT oligos attached to magnetic beads, reverse transcribed, made into Illumina libraries and sequenced using IlluminaHiSeq pair .. [more]
Center NameThe Wellcome Trust Sanger Institute