ERP014354 - DRA Search

Study Detail

Title	Utilizing 'Omics' technologies to identify and prioritize novel sources of resistance to the oomycete pathogen Phytophthora infestans in potato germplasm collections
Study Type	Other
Abstract	We have established and validated diagnostic RenSeq as a tool to quickly assess if resistant plants contain already characterized NB-LRR genes. Diagnostic RenSeq in resistant S. okadae accessions 7129, 7625, 3762 and a bulk of 20 resistant progeny confirmed the presence of full-length Rpi-vnt1.1 und .. [more]er stringent mapping conditions and corroborated Avr-vnt1 recognition and allele mining results in the accessions 7129 and 7625. In contrast, susceptible S. okadae accession 3761 and a bulk of 20 susceptible progeny lacked sequence homology in the 5’ end compared to the functional Rpi-vnt1.1 gene. Further evaluation of S. okadae accessions with late blight isolates that have a broad spectrum of virulence, has demonstrated that, although S. okadae accessions 7129, 7625 and 7629 contain functional Rpi-vnt1.1, they also carry a novel resistance gene. We have provided evidence that diagnostic RenSeq is a valuable tool to quickly establish if characterized resistance genes are present in wild Solanum species and propose its use for identifying orthologs with high sequence similarity and to aid R gene pyramiding and breeding. [less]
Description	We have established and validated diagnostic RenSeq as a tool to quickly assess if resistant plants contain already characterized NB-LRR genes. Diagnostic RenSeq in resistant S. okadae accessions 7129, 7625, 3762 and a bulk of 20 resistant progeny confirmed the presence of full-length Rpi-vnt1.1 und .. [more]er stringent mapping conditions and corroborated Avr-vnt1 recognition and allele mining results in the accessions 7129 and 7625. In contrast, susceptible S. okadae accession 3761 and a bulk of 20 susceptible progeny lacked sequence homology in the 5’ end compared to the functional Rpi-vnt1.1 gene. Further evaluation of S. okadae accessions with late blight isolates that have a broad spectrum of virulence, has demonstrated that, although S. okadae accessions 7129, 7625 and 7629 contain functional Rpi-vnt1.1, they also carry a novel resistance gene. We have provided evidence that diagnostic RenSeq is a valuable tool to quickly establish if characterized resistance genes are present in wild Solanum species and propose its use for identifying orthologs with high sequence similarity and to aid R gene pyramiding and breeding. [less]
Center Name	The James Hutton Institute, Scotland, United Kingdom

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