| Title | Genome sequencing of a Mobilitalea sibirica strain P3M-3T, novel anaerobic, mesophilic, halotolerant polysaccharide-degrading bacterium isolated from a microbial mat (Tomsk region, Russia). |
| Study Type | Other |
| Abstract |
Mobilitalea sibirica strain P3M-3T is a novel anaerobic, mesophilic, halotolerant bacterium inhabiting in microbial mat formed under the flow of hot water emerging from a 2775 m-deep well in Tomsk region (western Siberia, Russia). The cells of the novel strain are motile spore-forming straight or cu .. [more]rved rods with a Gram-positive type of cell wall. The novel isolate grew at 25-47°C (optimum 37°C), pH 6.6 -9.4 (optimum 7.0 – 7.5) and salinity 0 - 85 g/l of NaCl (optimum 15 g/l). The isolate was able to ferment a variety of mono-, di- and polysaccharides, including microcrystalline cellulose. 16S rRNA gene-based phylogenetic analysis showed that strain P3M-3T was a member of family Lachnospiraceae (phylum Firmicutes), whose representatives are also found in Clostridium cluster XIVa. 16S rRNA gene sequence similarity with Clostridium jejuense HY-35-12T, the closest relative, was 93.9 %. Strain P3M-3T shared several common features with other Clostridium-species: it was a rod-shaped, spore-forming, motile bacterium with a Gram-positive type of cell wall, was strictly anaerobic and neutrophilic, was able to grow chemoorganotrophically and not able to use electron acceptors. However, there were several genus-level discriminative features of strain P3M-3T: it was halotolerant and did not grow on fructose, galactose or sucrose. The differences between strain P3M-3T and its closest relative included the inability to grow on starch, galactose, sucrose, arabinose and mannitol. In addition, the novel isolate formed ethanol during fermentation. This study is aimed to give genomic and phylogenetic insights into these important metabolic features of M. sibirica. [less]
|
| Description |
Mobilitalea sibirica strain P3M-3T is a novel anaerobic, mesophilic, halotolerant bacterium inhabiting in microbial mat formed under the flow of hot water emerging from a 2775 m-deep well in Tomsk region (western Siberia, Russia). The cells of the novel strain are motile spore-forming straight or cu .. [more]rved rods with a Gram-positive type of cell wall. The novel isolate grew at 25-47°C (optimum 37°C), pH 6.6 -9.4 (optimum 7.0 – 7.5) and salinity 0 - 85 g/l of NaCl (optimum 15 g/l). The isolate was able to ferment a variety of mono-, di- and polysaccharides, including microcrystalline cellulose. 16S rRNA gene-based phylogenetic analysis showed that strain P3M-3T was a member of family Lachnospiraceae (phylum Firmicutes), whose representatives are also found in Clostridium cluster XIVa. 16S rRNA gene sequence similarity with Clostridium jejuense HY-35-12T, the closest relative, was 93.9 %. Strain P3M-3T shared several common features with other Clostridium-species: it was a rod-shaped, spore-forming, motile bacterium with a Gram-positive type of cell wall, was strictly anaerobic and neutrophilic, was able to grow chemoorganotrophically and not able to use electron acceptors. However, there were several genus-level discriminative features of strain P3M-3T: it was halotolerant and did not grow on fructose, galactose or sucrose. The differences between strain P3M-3T and its closest relative included the inability to grow on starch, galactose, sucrose, arabinose and mannitol. In addition, the novel isolate formed ethanol during fermentation. This study is aimed to give genomic and phylogenetic insights into these important metabolic features of M. sibirica. [less]
|
| Center Name | Immanuel Kant Baltic Federal University |
