Study Detail

Title3'-end targeted sequencing of 6 control and 6 TDP-43 KD samples in HEK293
Study TypeTranscriptome Analysis
Abstract HEK293 FlpIn T-Rex cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) with 10% fetal bovine serum (FBS), supplemented with 3 µg/ml blasticidine and 50 µg/ml zeocin. For the siRNA-induced knockdown of TDP-43, 20 nM of TDP-43 stealth siRNA was mixed with 10 µL of RNAiMAX following the ma .. [more]
Description HEK293 FlpIn T-Rex cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) with 10% fetal bovine serum (FBS), supplemented with 3 µg/ml blasticidine and 50 µg/ml zeocin. For the siRNA-induced knockdown of TDP-43, 20 nM of TDP-43 stealth siRNA was mixed with 10 µL of RNAiMAX following the ma .. [more]
Center NameInstitute of Molecular Life Sciences University of Zurich