Study Detail

TitlePrenatal overexposure to the synthetic glucocorticoid dexamethasone (Dex) in rats reduces birthweight in the first generation (F1) and second generation (F2), including through the male line. We hypothesised that the mechanism for this would likely be epigentic perturbations in the male germline. DNA methylation profiling of F1 male germ cells at E19.5 and small RNA expression and Chromatin Immunoprecipitation (ChIP) sequencing for H3K4me1, H3K4me3, H3K27me3 and H3K9me3 on F1 sperm were used to test this hypothesis. No differences in DNA methylation, histone modifications or small RNA expression were observed, suggesting non-epigentic mechanisms may be responsible for the transmission of programmed effects in this model.
Study TypeOther
Abstract Background. Early life exposure to adverse environments affects cardiovascular and metabolic systems in the offspring. These “programmed effects” are transmissible to a second generation through both male and female lines, suggesting germline transmission. We have previously shown that prenatal over .. [more]
Description Background. Early life exposure to adverse environments affects cardiovascular and metabolic systems in the offspring. These “programmed effects” are transmissible to a second generation through both male and female lines, suggesting germline transmission. We have previously shown that prenatal over .. [more]
Center NameDepartment of Biochemistry