Study Detail

TitleSmall RNA and total RNA sequencing of C. elegans with mutations in the hrde-1 and emb-4 genes
Study TypeTranscriptome Analysis
Abstract Synchronised animals were grown to young adult stage at 20C on HB101 seeded NGM plates. Animals were harvested and washed 3X in M9 buffer. Settled animals were mixed with Trisure reagent, bead beaten using zirconia beads, and the RNA is extracted using chloroform. For total RNA sequencing, Illumina .. [more]
Description Synchronised animals were grown to young adult stage at 20C on HB101 seeded NGM plates. Animals were harvested and washed 3X in M9 buffer. Settled animals were mixed with Trisure reagent, bead beaten using zirconia beads, and the RNA is extracted using chloroform. For total RNA sequencing, Illumina .. [more]
Center NameGURDON INSTITUTE