| Title | In situ Hi-C study of chromatin conformation during early Drosophila development |
| Study Type | Other |
| Abstract |
In this experiment we examined how chromatin is structured during early Drosophila melanogaster development. We performed in situ Hi-C for hand-sorted non-mitotic embryos at nuclear cycle number 12, 13 and 14, and for embryos at 3-4 hours post fertilisation. During this time in development, the zygo .. [more]tic genome is activated and zygotic transcription is taking place for the first time. To assess the impact of transcription on chromatin structure we use the transcription inhibitor alpha-amanitin. [less]
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| Description |
In this experiment we examined how chromatin is structured during early Drosophila melanogaster development. We performed in situ Hi-C for hand-sorted non-mitotic embryos at nuclear cycle number 12, 13 and 14, and for embryos at 3-4 hours post fertilisation. During this time in development, the zygo .. [more]tic genome is activated and zygotic transcription is taking place for the first time. To assess the impact of transcription on chromatin structure we use the transcription inhibitor alpha-amanitin. [less]
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| Center Name | MAX PLANCK INSTITUTE FOR MOLECULAR BIOMEDICINE |
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