Study Detail

Study TypeTranscriptome Analysis
Abstract RNAseq data was generated from trophoblast stem cells (TSCs) mutant for 3 selected genes of the DMDD consortium (Bap1, Crb2, Nubpl) and corresponding empty-vector control clones.
Description Total RNA was extracted from 5 knockout TSC clones ("HOM") for each gene and 3 corresponding empty-vector clones ("WT") each using Trizol reagent. Time points chosen were "0D" which means TSCs in the stem cell state grown under standard TSC conditions (Tanaka et al., Science 1998), and "3D" correspo .. [more]
Center NameThe Wellcome Trust Sanger Institute