Study Detail

TitleModified iCLIP sequencing of HEK293 cells with antibody PTBP1
Study TypeWhole Genome Sequencing
Abstract For modified iCLIP experiment, 4SU was used for crosslinking as described in published protocol (citation is bellow) and the RNase conditions were optimised to ensure efficient RNase I-dependent fragmentation. In detail, HEK293T cells were grown on 10 cm 2 dishes, incubated for 8 h with 100 µM 4SU a .. [more]
Description For modified iCLIP experiment, 4SU was used for crosslinking as described in published protocol (citation is bellow) and the RNase conditions were optimised to ensure efficient RNase I-dependent fragmentation. In detail, HEK293T cells were grown on 10 cm 2 dishes, incubated for 8 h with 100 µM 4SU a .. [more]
Center NameDEPARTMENT OF MOLECULAR NEUROSCIENCE AT THE UCL INSTITUTE OF NEUROLOGY. HTTP://ULELAB.INFO/