ERP114480
Study Detail
| Title | RNA-seq in Raji cells with inducible BZLF1 prior to and after induction of EBV's lytic cycle by doxycycline |
| Study Type | Transcriptome Analysis |
| Abstract | (i) RNA transcripts of Raji iBZLF1 cells (https://www.biorxiv.org/content/10.1101/573659v1) were compared between non-induced cells and cells induced with doxycycline (100 ng/ ml) for 6 h. Human (Hg19) as well as viral (EBV, Raji KF717093.1) transcripts were analyzed. Artificial ERCC Spike-ins (Ther .. [more]mo Scientific) were added to selected samples to control for global changes of RNA levels. (ii) For control purposes, the RNA transcripts in Raji cells equipped with a doxycycline-controlled truncated BZLF1 allele lacking its transactivation domain (Raji iBZLF1 AD-truncated) were prior to and after induction for 6 h. (iii) For another control, the RNA transcripts of parental, unmodified Raji cells were analyzed prior to and after doxycycline induction for 6 h. All experiments were performed as triplicates. [less] |
| Description | (i) RNA transcripts of Raji iBZLF1 cells (https://www.biorxiv.org/content/10.1101/573659v1) were compared between non-induced cells and cells induced with doxycycline (100 ng/ ml) for 6 h. Human (Hg19) as well as viral (EBV, Raji KF717093.1) transcripts were analyzed. Artificial ERCC Spike-ins (Ther .. [more]mo Scientific) were added to selected samples to control for global changes of RNA levels. (ii) For control purposes, the RNA transcripts in Raji cells equipped with a doxycycline-controlled truncated BZLF1 allele lacking its transactivation domain (Raji iBZLF1 AD-truncated) were prior to and after induction for 6 h. (iii) For another control, the RNA transcripts of parental, unmodified Raji cells were analyzed prior to and after doxycycline induction for 6 h. All experiments were performed as triplicates. [less] |
| Center Name | Research Unit Gene Vectors Helmholtz Zentrum Munchen German Research Center for Environmental Health and German Center for Infection Research (DZIF), Partner site Munich, Germany |
SRA Links
| Url Link | E-MTAB-7820 in ArrayExpress |
Navigation
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Submission | ERA1800239 |
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Experiment | ERX3279699 |
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| ERX3279700 |
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| ERX3279701 |
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| ERX3279702 |
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| ERX3279703 |
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| ERX3279704 |
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| ERX3279705 |
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| ERX3279706 |
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| ERX3279707 |
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| ERX3279708 |
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| ERX3279709 |
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| ERX3279710 |
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| ERX3279711 |
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| ERX3279712 |
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| ERX3279713 |
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| ERX3279714 |
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| ERX3279715 |
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| ERX3279716 |
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Sample | ERS3339300 | ||
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| ERS3339317 |