ERP114841

Title	ATAC-seq of primary mammalian aortic endothelial cells before and after TNFa stimulation
Study Type	Other
Abstract	To identify conserved TNFa-induced changes in chromatin-accessibility in mammals, we performed ATAC-seq in primary vascular endothelial cells (ECs) isolated from the aortas of human (HAEC), mouse (MAEC) and cow (BAEC), before and after TNFa. We overlay our data with multi-species NF-?B binding data .. [more]and identify multiple modes of NF-?B-chromatin interactions that are conserved during mammalian TNFa response. Our cross-species approach identifies conserved changes in chromatin-accessibility at NF-?B binding sites that are disease-relevant and essential during mammalian acute inflammation. [less]
Description	To identify conserved TNFa-induced changes in chromatin-accessibility in mammals, we performed ATAC-seq in primary vascular endothelial cells (ECs) isolated from the aortas of human (HAEC), mouse (MAEC) and cow (BAEC), before and after TNFa. We overlay our data with multi-species NF-?B binding data .. [more]and identify multiple modes of NF-?B-chromatin interactions that are conserved during mammalian TNFa response. Our cross-species approach identifies conserved changes in chromatin-accessibility at NF-?B binding sites that are disease-relevant and essential during mammalian acute inflammation. [less]
Center Name	The Hospital for Sick Children, Genetics and Genome Biology University of Toronto, Department of Molecular Genetics

Url Link

DRASearch