Study Detail

TitleSequence Level Analysis of Double Minute Chromosomes in the Ovarian Cancer Cells Yields Insights into Their Complicated Molecular Architecture
Study TypeCancer Genomics
Abstract We established optimal conditions for two-dimensional pulsed field gel electrophoresis and purified DMs from ovarian cancer cell line UACC-1598. The DMs in the cancer cells are heterogeneous in size (1–3 Mb), and the purified DNA molecules were confirmed as intracellular DMs using fluorescence in si .. [more]
Description Double minute chromosomes (DMs), the autonomously replicating extra-chromosomes, are frequently associated with gene amplification in tumors. However, their molecular architecture and the underlying mechanisms leading to their formation and function are largely unknown.In our study, the rearrangemen .. [more]
Center NameHarbin Medical University