Study Detail

TitleDRIP and DRIVE-seq reads in human Ntera2 cells
Study TypeOther
Abstract To sequence RNA:DNA hybrids in the human genome using the DRIP and DRIVE methods (DNA:RNA ImmunoPrecipitation,DNA:RNA In Vitro Enrichment, Ginno et al. 2012).
Description Extracted Ntera2 genomic DNA was fragmented with 5 restriction enzymes (EcoRI, HindIII, BsrGI, XbaI, SspI) and RNA:DNA hybrids enriched using the S9.6 monoclonal antibody or MBP-RnaseH1 mutant. Eluate was then sonicated and ligated to Adapters for sequencing on the Illumina GAII and HiSeq platforms .. [more]
Center NameUC Davis