Study: JGAS00000000038




TitleParent-of-origin dependent DNA methylation and gene expression in the human placenta
AbstractDNA methylation is globally reprogrammed after fertilization and the parental genomes have similar DNA methylation profiles after implantation except at the germline differentially methylated regions (gDMRs). We, and others, have previously shown that there are a large number of transient gDMRs in human blastocysts, whose differential methylation is lost in embryonic tissues after implantation. In this study we employed genome-wide allelic DNA methylation analyses of highly purified trophoblast cells from human placentas. ~40% of the transient-in-embryo gDMRs maintained their parent-of-origin-dependent biased allelic methylation. RNA sequencing-based allelic expression analyses revealed that some of the placental-specific gDMRs were associated with novel imprinted genes. This approach identified the first examples of X-linked gDMRs specific to the placenta. These findings highlight the unique regulation of allelic DNA methylation in the human placenta, which is important for understanding normal placental development and the pathogenesis of pregnancy complications.
Study Type 1
Study TypeWhole Genome Sequencing
Study Type 2
Study TypeExome Sequencing
Study Type 3
Study TypeTranscriptome Sequencing
Study Type 4
Study TypeOther
New Study TypeBisulfite Sequencing

Study Attribute

NBDC Numberhum0040
Registration date2015-09-17
Submitting organizationDepartment of Informative Genetics, Environment and Genome Research Center, Tohoku University Graduate School of Medicine
Accession Title Dataset type Data objects Policy
JGAD00000000038 Parent-of-origin dependent DNA methylation and gene expression in the human placenta Whole genome sequencing 88 JGAP00000000001