Study: JGAS00000000065

Identifier

AccessionJGAS00000000065

Description

TitleSequencing cancer mutations in lung cancers using the long-read, portable sequencer
AbstractIn this study, we used a long-read and portable sequencer MinION to identify cancer mutations in lung adenocarcinoma cells. We performed cDNA amplicon sequencing for various types of mutations such as point mutations in EGFR and KRAS and oncogenic fusion transcripts in ALK and RET. Using the long reads of MinION, we also conducted mutation phasing of EGFR TKI-sensitive and resistant mutations, which would provide useful information to next thrapeutic approaches for cancers. Finally, we applied this method to identify mutation genotypes of eight clinical samples from Japanese lung adenocarcinomas. This method could provide new sequencing-based diagnostic approaches for lung cancers.
Study Type
Study TypeCase Set

Publication

DOI ID10.1093/dnares/dsx027
Publication Statuspublished
DB TypeDOI

Study Attribute

NBDC Numberhum0068
Registration date2016-08-16
Submitting organizationNational Cancer Center Research Institute
Principal InvestigatorTakashi Kono
Molecular Data TypeAmplicon Sequencing
PlatformMinION
VendorOxford nanopore
Comment
Primary PhenotypeCancer
PhenotypeLung adenocarcinoma
Dataset
Accession Title Dataset type Data objects Policy
JGAD00000000065 Amplicon sequencing of lung adenocarcinoma Amplicon sequencing 7 JGAP00000000001